Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANMQTIIDETIAEFLAENGYFYIMHRF----DEAARIPFIKKMKKRGLITSISVGVKKEEYSFIEKLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARK---PIIADGGIRTHGDIAKSVRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSAS------EFQKGEKRNV-EGKKIWIQHKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI 4MY9 Chain:E ((35-353)) ---LTFDDVLLVPAKSDVLPREVSVKTVLSESLQLNIPLISAGMDTVTEADMAIAMARQGGLGIIHKNMSIEQQAEQVDKVKR--SGGLLVGAAVGVTADAMTRIDALVKASV--DAIVLDTAHGHSQGVIDKVKEVRAKYPSLNIIAGNVATAEATKALIEAGANVVKVGIGPGSICTTRVVAGVGV--PQLTAVYDCATEARKHGIPVIADGGIKYSGDMVKALAAGAHVVMLGSMFAGVAESPGETEIYQGRQFKVYRGMGSVGAMEK--------LVPEGIEGRVPYKGPLADTVHQLVGGLRAGMGYCGAQDLEFLRE----------------

General information: TITO was launched using: RESULT: Template: 4MY9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1776 -44204 -24.89 -151.38 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain E : 0.76 3D Compatibility (PKB) : -24.89 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_4MY9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MY9-query.scw PDB file : Tito_Scwrl_4MY9.pdb: