Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGKRIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNHKVFGEALAILAGDGLLTGAFQLISMAHLGN-SPKLLLLQQLAVCAGSQGMVAGQAADIEGESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQ-PEEILLLLQRLAEHLGLAFQIRDDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEEDVFSFDGGLMRQMIKQFDVEK 4F62 Chain:B ((3-278)) MNLKQFSTYTQSRVDQYLEQQLSDYAPANQLHNAMRYSLF-GGKRIRPMLTYASAQLVGDISSL-TDASAAALESIHAYSLIHDDLPAM---------------FDEATAILAGDALQTFAFELLSNPTSAQPELAIKLIQELVVASGRNGMITGQMIDLSSEN--ISLAELEQMHVHKTGALIKASVRMGALSTGQVKPEQLAKLDAYAHAIGLAFQVQDDIIDL------------------KATYPKLLGLDGAKALVVRLHEQAIAQISEFGD---------------------

General information: TITO was launched using: RESULT: Template: 4F62.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1058 -73281 -69.26 -307.90 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -69.26 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_4F62.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F62-query.scw PDB file : Tito_Scwrl_4F62.pdb: