Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLVEKLRRKIMQSKTLFLLGLCTIFSTTVSALPLDSKGAKQRLSQAAKQKVFQGKIDLDALVSNDSNDLIVEYNIPSSSVSSGLERRSYIATNKKNLQTRFSRAGGVQVLRDYNNLPLAFYRISNREALIALLNDPNVKAVYPNRINRTTTTESLPLINQPQANTNGFTGEGSSVAVLDTGVNYLHSDFGCTAVNTPSSTCRVVYSFDSAP-DDGTLDDDGHGSNVSAIVS---------KVATKTKIIGIDVFRKVRSQGKWVSTAYDSDILAGINWAVNNAQTYNIKAVNLSLGVPGVKYTSECSDSSYGTAFANARAAGVVPVVASGNDAFP---DGISSPACVAGAVRVGAVYDSNIGGVSWGNPVKCSDPTTAADKVACFSNGGSLVTLLAPGAMITAG-----GYTMGGTSQATPHVAGAIALLRANSVSPTESIDQTISRLKATGKPITDSRTGLVFPRIDLLAATNGLTVN 1R0R Chain:E ((5-274)) -------------------------------------------------------------------------------------------------------------------------------------------------------PYGIPLIKADKVQAQGFKGANVKVAVLDTGIQASHPDLNVVG------------GASFVAGEAYNTDGNGHGTHVAGTVAALDNTTGVLGVAPSVSLYAVKVLNSS-------GSGSYSGIVSGIEWATTN----GMDVINMSLGGASG-------STAMKQAVDNAYARGVVVVAAAGNSGNSGSTNTIGYPAKYDSVIAVGAVDS--------------------NSNRASFSSVGAELEVMAPGAGVYSTYPTNTYATLNGTSMASPHVAGAAALILSKH--PNLSASQVRNRLSSTATYLGS-SFYYGKGLINVEAAAQ-----

General information: TITO was launched using: RESULT: Template: 1R0R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1778 6527 3.67 25.90 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain E : 0.65 3D Compatibility (PKB) : 3.67 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_1R0R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1R0R-query.scw PDB file : Tito_Scwrl_1R0R.pdb: