Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDLDTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNMEINHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSESRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAPWNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIGVFSPALDPFGNSAAGIQLLKDVVKELDADIFE 2PBY Chain:C ((6-307)) --QEELVRFVEEAKQYARYGKVADYIPALGKANPNELSIAIYTPD-DEVVSAGDVTVKVTLQSISKIIALALVLIDRGEDEVFHKVGMEP-----------------KPLNPMINAGALVVTSMIQGGSVSERLERLLAFVRRLAGNERISYSDEVARSEFETAFLNRSLCYFLKQHRIIDEDVEELMELYTKQCAIEMTCIDLARIGLVLALDGRDPHSSEPLMPLDVARICKTFMVTCGMYNSSGEFAIKVGIPAKSGVSGGILAAVPGRCGIGVFGPALDDKGNSLTGVKLLERLSKTYSLSI--

General information: TITO was launched using: RESULT: Template: 2PBY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1740 -191112 -109.83 -668.22 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.84 3D Compatibility (PKB) : -109.83 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_2PBY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PBY-query.scw PDB file : Tito_Scwrl_2PBY.pdb: