Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQVDIAALSP--QVVWQHFQTLCTIPRPSKHEQ------------QLREFLQNWAESRNLETYVDEVGNLIIRKNATPGKKHVSGVILQGHLDMVTQANTGTVHDFFKDPIRPVLE-EGWLIAKDTT--LGADNGIGVALALAVLDSNDIHGPIEVLLTVDEEAGMSGARLLETGVLKGKWLFNIDTEEWGELYLGCAGSIDVEVEQPLNYEPIPENLNIVNIQVAGLKGGHS------GVDIHLGRGNANVILARFLNQHLASLGGHLVEFTGGTARNAL--PREAVATIAISPNQLSSLEKLLAEYQAAWKEQLKGIDDNLQLSIQSTGAKVTEVINQQQQNEWLQALATSPYGVASMSQVLPDVVETSNNIGVVRLNREG-GKAILMVRSMVNQEAQDFAEKIQAHFSQFNIGSTLTPLVSGWTPNPDSAALKCLQQAYQNAFNIEPNLKVIHAGLECGIIAEHYPHLQMVSFGPDIQG----AHAPGERVKVDTVEKCWKLLVTALASVE--- 1LFW Chain:A ((1-468)) MDLNFKELAEAKKDAILKDLEELIAIDSSEDLENATEEYPVGKGPVDAMTKFLSFAKRDGF--DTENFANYAGRVNFGAGDK---RLGIIGHMDVVPAGE-----GWTRDPFKMEIDEEGRIYGRGSADDKGPSLTAYYGMLLLKEAGFKPKKKIDFVLGTNEETNWVGIDYYLKHEPTPDIVFSPDAEYP--IINGEQGIFTLEFSFKNDDTK-------GDYVLDKFKAGIATNVTPQVTRATISGPDLEAVKLAYESFLADKELDGSFEINDESADIVLIGQGAHASAPQVGKNSATFLALFLDQ-----------------YAFAGRDKNFLHFLAEVEHEDF----YGKKLGIFHHDDLMGDL---ASSPSMFDYEHAGKASLLNNVRYPQGTDPDTMIKQVLDKFSG-ILDVTYNGFEEPHYVPGSDPMVQTLLKVYEKQTGKPGHEVVIGGGTYGRLFE------RGVAFGAQPENGPMVMHAANEFMMLDDLILSIAIYAEAIYELTKDE

General information: TITO was launched using: RESULT: Template: 1LFW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2613 87789 33.60 201.81 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 33.60 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.355

(partial model without unconserved sides chains): PDB file : Tito_1LFW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LFW-query.scw PDB file : Tito_Scwrl_1LFW.pdb: