Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGKRIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNHKVFGEALAILAGDGLLTGAFQLISMAHLGN-SPKLLLLQQLAVCAGSQGMVAGQAADIEGESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQ-PEEILLLLQRLAEHLGLAFQIRDDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEEDVFSFDGGLMRQMIKQFDVEK 4F62 Chain:A ((3-278)) MNLKQFSTYTQSRVDQYLEQQLSDYAPANQLHNAMRYSLF-GGKRIRPMLTYASAQLVGDISSL-TDASAAALESIHAYSLIHDDLPAMDN-------PTCHIQFDEATAILAGDALQTFAFELLSNPTSAQPELAIKLIQELVVASGRNGMITGQMIDLSSENKNISLAELEQMHVHKTGALIKASVRMGALSTGQVKPEQLAKLDAYAHAIGLAFQVQDDIIDLT----------------NKATYPKLLGLDGAKALVVRLHEQAIAQISEFGD---------------------

General information: TITO was launched using: RESULT: Template: 4F62.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1157 -74265 -64.19 -297.06 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -64.19 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_4F62.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F62-query.scw PDB file : Tito_Scwrl_4F62.pdb: