Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRISDQVVMKLQALIEERHMKKGDRLPAERQLATSLGVSRPSLREAIQQLNSQGVLISRRGDGTYIQQLPEQWPQQLIVNPISNLI----EEDPLYRFDVQEARLVLEGGTAWYAALRSTPEDRAKIHHYFNEISRHQN----------------------A------------GDSAQAAVADAEFHLAIAEASHNVVLIQMMRSLFDLLQYNVLLGRKKVYNDPVNGDLLSEQHFQVMDAIDRKDPEAARQAVCGHIEFVINHVRSLDEDEARQKRANRLNRVDSK 4P96 Chain:B ((7-269)) -SPAGFAEKYIIESIWNGRFPPGSILPAERELSELIGVTRTTLREVLQRLARDGWLTIQHGKPTKVNQFM----ETSGLHILDTLMTLDAENATSIVEDLLAARTNISPIFMRYAFKLNKESAERIMINVIESCEALVNAPSWDAFIAASPYAEKIQQHVKEDSEKDELKRQEILIAKTFNFYDYMLFQRLAFHSGNQIYGLIFNGLKKLYDRVGSYY----FSNPQARELAMEFYRQLLAVCQSGEREHLPQVIRQYGIASGHIWNQMKMT----------------

General information: TITO was launched using: RESULT: Template: 4P96.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 801 -45232 -56.47 -201.03 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -56.47 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_4P96.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P96-query.scw PDB file : Tito_Scwrl_4P96.pdb: