Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEIVLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQMKNI-TDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH 1SJN Chain:C ((16-113)) -----------------LPSRAHDGDAGVDLYSAEDVELAPGRRALVRTGVAVAVPFGMVGLVHPRSGLATRVGLSIVNSPGTIDAGY------RGEIKVALINLDPAAPIVVHRGDRIAQ---------------------------

General information: TITO was launched using: RESULT: Template: 1SJN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 408 -40089 -98.26 -413.28 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : -98.26 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_1SJN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SJN-query.scw PDB file : Tito_Scwrl_1SJN.pdb: