Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYAT---DKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLTMHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIGDYDYK--VLMHY 4X45 Chain:B ((8-179)) -LVEQRIRSFPDFPTPGVVFRDISPVLKDPASFRAAIGLLARHLKATHGGRIDYIAGLDSRGFLFGPSLAQELGLGCVLIRKRGKLPGPTLWASYS----KAELEIQKDALEPGQRVVVVDDLLATGGTMNAACELLGRLQAEVLECVSLVELTSLKGREKLA--PVPGFSLLQY

General information: TITO was launched using: RESULT: Template: 4X45.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 807 -32536 -40.32 -199.61 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -40.32 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_4X45.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X45-query.scw PDB file : Tito_Scwrl_4X45.pdb: