Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLYSLARPMLFSLAPERAHELTLSMLDKAHKLGIMRQTVEAKPTTCMGIEFPNPVGLAAGLDKNGAHIDALAGLGFGFIEIGTITPHPQSGNPKPRLFRIPEAKAIINRMGFNNDGVDKLIENVKASKFR---------GILGINIGKNADTPVEKAVDDYLICLEKVYNYASYITVNISSPNTKNLRSLQSGDALTELLQALKARQLELAEQYN------------HYVPLVL-KVAPDLTAEDVEFISAQLLDFKIDGLIVTNTTLSREGVENLPYGNESGGLSGAPVFEKSTECLRLFAQTLKGQISLIGVGGILSGEQAAAKQQAGATLVQIYSGLIYTGPTLVKQCVEAMT 3SFK Chain:A ((71-366)) -------------------------------------------TNIKHLDFINPFGVAAGFDKNGVCIDSILKLGFSFIEIGTITPRGQTGNAKPRIFRDVESRSIINSCGFNNMGCDKVTENLILFRKRQEEDKLLSKHIVGVSIGKNKDTV--NIVDDLKYCINKIGRYADYIAINVSSP-------NQEAGKLKNIILSVK-EEIDNLEKNNIMNDEFLWFNTTKKKPLVFVKLAPDLNQEQKKEIADVLLETNIDGMIISNTTTQINDIK--SFENKKGGVSGAKLKDISTKFICEMYNYTNKQIPIIASGGIFSGLDALEKIEAGASVCQLYSCLVFNG------------

General information: TITO was launched using: RESULT: Template: 3SFK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1417 21858 15.43 81.87 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 15.43 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_3SFK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SFK-query.scw PDB file : Tito_Scwrl_3SFK.pdb: