Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLSKKTIIALVVAAIALILAICVFVWKKPQSSSSSQENSASTQDHPVEKAEVLPYLNLTETKANYAVPFCEKKNCIDVDIQTIKTQDAWLNSWIEKNQAKVIQAQINLKKDLSLQQAINAYVKKSDEWQDKYSKNRAYELHIRTRIASQRNQYVLLQLALDSKQEEITIKDRYYFFVADRKLHKNLTLFDVLKKDQQPTVHQIVQTAYQDWLKKQTADVKKQAPKTLYWGQADWFFDGEGVGLHYQANQITKDAPQLDIYLTTEQTKKILQPQVYEQMF 2MC7 Chain:A ((1-30)) MNRSPDKIIALIFLLISL-LVLCLALWQIVF---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2MC7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 10 -1562 -156.20 -52.07 target 2D structure prediction score : 0.90 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -156.20 2D Compatibility (Sec. Struct. Predict.) : 0.90 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.791

(partial model without unconserved sides chains): PDB file : Tito_2MC7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MC7-query.scw PDB file : Tito_Scwrl_2MC7.pdb: