Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MTSYANLLKPLHLGFTTIKNRVVMGSMHTGLEDRFFNYPKLAAYFEERAKGGVGLIVTGGISPNRQGWLAPAGGT-MNSLFDIPQHRLVTHAVHKHGSKILMQILHAGRYGYQ------PFVVSSSPI-KSPISPFKPRQLSEKNILSTIEDYAQCADIAKKAGYDGVEIMGSEGY-LINQFLSRHVNQRTDRWGGEIENRMRFPVEIVKAIRAKVGEKFIICFRLSLLDLVHDGNTMQEVVTVAKALEKAGVTLLNTGIGWHEARVPTIVTSVPRAAFVDYTAHVKQHISIPVIASNRINMPETAEEILATGKADMIQMARPLLADAFWVNKTATNRVDEINTCIACNQACLDHAFKNKRVSCLVNPRAGHETELVYL-----KTKQPKRIAVVGGGVAGMSAATVAASRGHAVTLFEANADVGGQFNFAKVVPGKEEFHETIRYFKVQLQK-TGVDVRLNTRVNREQLEREGFDEVIVATGVVPR--------ALKIEGSNSPQVLSYAQVLQGAE-VGRKVAVIGAGG--IGFDVSEFLLKPPHQPQSQPLAEWQREWGVDPDPNYVSEGGMQPPVVEPAIREIYLLQRKTTPLGVGLGKTS-GWVHRAQLKKHGVRMLRGVQYKAVTDEGLWIE--HNGQDQLLRVDTVVVCAGQES----VKDLMPKEGESTIANYHIIGGAKLAAELDAKRAIKEGAELAAQL-------------------- 3K30 Chain:A ((7-690)) VAAPYDVLFEPVQIGPFTTKNRFYQVPHCNGMGYR--DPSAQASMRKIKAEGGWSAVCTEQVEIHATSDIAPFIELRIWDDQDLPALKRIADAIHEGGGLAGIELAHNGMNAPNQLSRETPLGPGHLPVAPDTIAPIQARAMTKQDIDDLRRWHRNAVRRSIEAGYDIVYVYGAHGYSGVHHFLSKRYNQRTDEYGGSLENRMRLLRELLEDTLDECAGRAAVACRITVEEEIDGGITREDIEGVLRELGE-LPDLWDFAMGSWEGDSVT-SRFAPEGRQEEFVAGLKKLTTKPVVGVGRFTSPDAMVRQIKAGILDLIGAARPSIADPFLPNKIRDGRLNLIRECIGCN-ICVSGDLTMSPIRCTQNPSMGEEWRRGWHPERIRAKESDARVLVVGAGPSGLEAARALGVRGYDVVLAEAGRDLGGRVTQESALPGLSAWGRVKEYREAVLAELPNVEIYRESPMTGDDIVEFGFEHVITATGATWRTDGVARFHTTALPIAEGMQVLGPDDLFAGRLPDGKKVVVYDDDHYYLGGVVAELLAQKGY--------------------------------------EVSIVTPGA-QVSSWTNNTFEVNRIQRRLIENGVARVTDHAVVAVGAGGVTVRDTYASIERELECDAVVMVTARLPREELYLDLVARRDAGEIASVRGIGDA--WAPGTIAAAVWSGRRAAEEFDAVLPSNDEVPFRREVTQLA

General information: TITO was launched using: RESULT: Template: 3K30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3957 65383 16.52 103.78 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 16.52 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_3K30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3K30-query.scw PDB file : Tito_Scwrl_3K30.pdb: