Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADLHTSVFGIELDTPIIQAPSAAQGLAHE-KGEADTAKGVAAAGSIFS--ISTYANTTIKDAADAAPGAPQ--FFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFPLPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSPEDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKR-----VPVIFDSGVRRGEHVFKALASGADLVAIGRPVIYGLNLVGQKE 1FCB Chain:B ((140-444)) ----AWAYYSSGANDEVTHRENHNAYHRIFFKPKILVDVRKVDISTDMLGSHVDVPFYVSATALCKLGNPLEGEKDVARGCGQGVTKVPQMISTLASCSPEEIIEAAPSDKQIQWYQLYVNSDRKITDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFS-------------NVEESQGASRALSKFIDPS---LTWKDIEELKKKTKLPIVIKGVQRTEDVIKAAEIGVSGVVLSNHGGRQLDFSRAPIEVLAETMPILEQRNLKDKLEVFVDGGVRRGTDVLKALCLGAKGVGLGRPFLYANSCYGR--

General information: TITO was launched using: RESULT: Template: 1FCB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1453 45600 31.38 161.13 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : 31.38 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_1FCB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FCB-query.scw PDB file : Tito_Scwrl_1FCB.pdb: