Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSCYLITGGAGFIGSTLANHLG-EE--HVVIVVDDLSMGKV-EN---LDMTKNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMILELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYCHLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSRDFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTL-EYKEERSGDIHDSLADITKIQS-IGYQPKYGILSGMTNYLKTEIN 2HUN Chain:B ((3-312)) SMKLLVTGGMGFIGSNFIRYILEKHPDWEVINIDKLGYGSNPANLKDLEDDPRYTFVKGDVADYELVKELV--RKVDGVVHLAAESHVDRSISSPEIFLHSNVIGTYTLLESIRRENP-EVRFVHVSTDEVYGDILKGSFTENDRLMPSSPYSATKAASDMLVLGWTRTYNLNASITRCTNNYGPYQFPEKLIPK--------TIIRAS-LGLKIPIYGTGKNVRDWLYVEDHVRAIELVLLKG-ESREIYNISAGEEKTNLEVVKIILRLMGKGEELIELVEDRPGHDLRYSLDSWKITRDLKWRPKYTFDEGIKKTIDWYL-

General information: TITO was launched using: RESULT: Template: 2HUN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1751 10047 5.74 33.38 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : 5.74 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_2HUN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HUN-query.scw PDB file : Tito_Scwrl_2HUN.pdb: