Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIYDYIVIGGGSGGI-ASANRAGMHGAKVLLIEANE---------LGGTCVNVGCVPKKVMWQASTILETIERDASSYGIQADLKNV--DFAGLVERREKYIDFLHGAYQRGL-DSNHVEAIKGYATFIDSQTIEVNG---------ETYRAPKILIATGGRASKMTIPGGEYAIDSNGFFELTELPKRAIVLGAGYIAAELSGVFRG---LGSEVMWAYRKERPLRTFDKMLSDNLIQMYQEAGIKTYAHHIAKEITKNND-EYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVELDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDYKLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEM-KLICVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR 5EBK Chain:A ((23-492)) -RAYDLVVLGAGSGGLEAGWNAAVTHKKKVAVVDVQATHGPPLFAALGGTCVNVGCVPKKLMVTGAQYMDLI-RESGGFGWEMDRESLCPNWKTLIAAKNKVVNSINESYKSMFADTEGLSFHMGFGALQDAHTVVVRKSEDPHSDVLETLDTEYILIATGSWPTRLGVPGDEFCITSNEAFYLEDAPKRMLCVGGGYIAVEFAGIFNGYKPCGGYVDLCYRGDLILRGFDTEVRKSLTKQLGANGIRVRTNLNPTKITKNEDGSNHVHFNDGTEEDYDQVMLAIGRVPRSQALQLDKAGVRTGKNGAVQVDAYSKTSVDNIYAIGDVTNRVMLTPVAINEGAAFVETVFGGKPRA-TDHTKVACAVFSIPPIGTCGMTEEEAAKNY--ETVAVYASSFTPLMHNISGSKHKEFMIRIITNESNGEVLGVHMLGDSAPEIIQSVGICMKMGAKISDFHSTIGVHPTSAEELCSMR

General information: TITO was launched using: RESULT: Template: 5EBK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2590 7020 2.71 15.85 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 2.71 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_5EBK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EBK-query.scw PDB file : Tito_Scwrl_5EBK.pdb: