Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFVLGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQPDVSLRVNIKPAVDAQVQVLSEA-GVGISDFNKGNIKARQRMITQYAVAGERAGAVLGTDHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGKPMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK 1KQP Chain:B ((1-271)) -SMQEKIMRELHVKPSIDPKQEIEDRVNFLKQYVKK-TGAKGFVLGISGGQDSTLAGRLAQLAVESIREE--GGDAQFIAVRLPHGTQQDEDDAQLALKFIKPDKSWKFDIKSTVSAFSDQYQQETGDQLTDFNKGNVKARTRMIAQYAIGGQEGLLVLGTDHAAEAVTGFFTKYGDGGADLLPLTGLTKRQGRTLLKELGAPERLYLKEPTADLLDEKPQQSDETELGISYDEIDDYLEGKEVSAKVSEALEKRYSMTEHKRQVPASMFDDWWK

General information: TITO was launched using: RESULT: Template: 1KQP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1361 1302 0.96 4.82 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain B : 0.88 3D Compatibility (PKB) : 0.96 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_1KQP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KQP-query.scw PDB file : Tito_Scwrl_1KQP.pdb: