Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MARMAKVVELPSIAKIDKSKGKISAQLTQALREAVQSGDLQPGDPLPSSRELALTLGVARGTIIEAYDQLLAEGVFDSQPRTGTYVSHALVKTSAIQHSSKENKPNSISLNKSARSYAKVLDEFKPLPHVPFAVSVPIARTQPSDIWRKFGNKFRSRGAGVPSGYGEPQGVLSLRIAIADYVRRSRSVH-CEPEQIVITPSIQQSLYICNQILFEAKDEVWVEDPAYRGTTAIFEHTVQNIRMVRVPVDEEGIQIETGIKL-S--------KQASAAFVTPSHQYPLGMPMSLARRTALLAWAKQQNAWIIEDDYDSELRYNGQPFPSLQGMAP-DQAIYLGTFSKVLFPSLRLGYAVLPKELVAPFCGLRVLIDRHPPAADQHVLAAFIQEGYLERHIRRTRNVYAEVREYIIGLIEKYIP--QELAWLQLGDQGMHMVLWLAQHINDIDVASSAVDAGIAIKAISPTFSKERRRSGLIVGLGDFEPELMQQAIKKLSKIIQQHAKSM 1VP4 Chain:B ((43-420)) ------------------------------------------------------------------------------------------------------------------------------KDAISFGGGVPDPETFPRKELAEIAKEIIEKEYHYTLQYSTTEGDPVLKQQILKLLERMYGITGLDEDNLIFTVGSQQALDLIGKLFLDDESYCVLDDPAYLGAINAFRQYLA--NFVVVPLEDDGMDLNVLERKLSEFDKNGKIKQVKFIYVVSNFHNPAGVTTSLEKRKALVEIAEKYDLFIVEDDPYGALRYEGETVDPIFKIGGPERVVLLNTFSKVLAPGLRIGMVAGSKEFIRKIVQAKQSADLCSPAITHRLAARYLERYDLLEQLKPTIELYRRKRTVMLNALEEYFSDIPGVKWV-KSEGGLFIWLTLPEGFDTWEMFEYAKRKKVFYVPGRVFKVYDEPSPSMRLSFCLPPDEKIVEGIKRLREVVLEYGK--

General information: TITO was launched using: RESULT: Template: 1VP4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2044 -18185 -8.90 -49.82 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -8.90 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_1VP4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VP4-query.scw PDB file : Tito_Scwrl_1VP4.pdb: