Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTDTALIMALPNESKGLFE-QA--------------------GIEVHYSGIGKINAAFKAFEVIQKTGCKTLINLGTAGSSS--FNRHDLVEIKTFVQRDMDVSPLGFEVGVTP-----LDDHLAAEIHLQT----HFADLPKGICGTGDSFETGQPKV-------ACDVVDMEGYALAKVCHKLGVRLISVKYITDGADDTAHLDWEENLLLGAQKLLALYQNHF 4JWT Chain:A ((22-245)) -SMKIAIMGAMPEEISPILEKIGSYKSTSYAGNKYYEATYQGVELVIAYSKIGKVFSALSAATMIEHFGATKLLFSGVAGAISTNLKVGDLIVATKLSQHDLDITAFGHPYGYVPEGSVFVEADKDMIELSKKVALEMGKSVQEGIIATGDQFVANEERKNWIGTTFGADALEMEGGSVGVVCNALNIPFFILRSISDAADMDASFSFDEFLESSAKESAEFIMK--

General information: TITO was launched using: RESULT: Template: 4JWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 919 -139 -0.15 -0.75 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -0.15 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.440

(partial model without unconserved sides chains): PDB file : Tito_4JWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JWT-query.scw PDB file : Tito_Scwrl_4JWT.pdb: