Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPELPEVETVCRGLEKLIIGKKISSIEIRYPK-MIKT-DLEEFQRELPSQIIESMGRRGKYLLFYLT-DKVLISHLRMEGKYFYYPDQ-------------GPERKHAHVFFHFEDGGTLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSHLLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGSTIRTYTNAFGEDGSMQDFHQVYDKTGQECVR--CGTIIEKIQLGGRGTHFCPNCQRRD 3TWK Chain:A ((2-279)) -PELPEVEAARRAIEENCLGKKIKRVIIADDNKVIHGISPSDFQTSILGKTIISARRKGKNLWLELDSPPFPSFQFGMAGAIYIKGVAVTKYKRSAVKDSEEWPSKYSKFFVELDDGLELSFTDKRRFAKVRLLANPT--SVSPISELGPDALLEPMTVDEFAESLAKKKITIKPLLLDQGYISGIGNWIADEVLYQARIHPLQTASSLSKEQCEALHTSIKEVIEKAVEVDADSS-----------QFPSNWIFHNREKKPGKAFVDGKKIDFITAGGRTTAYVPELQKLY

General information: TITO was launched using: RESULT: Template: 3TWK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1333 34831 26.13 133.96 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 26.13 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.429

(partial model without unconserved sides chains): PDB file : Tito_3TWK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TWK-query.scw PDB file : Tito_Scwrl_3TWK.pdb: