Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENDMKKISLVIAASTMSLSVFAAAPITNKSPAKDQFSYSYGYLMGRNNTDALTDLNLDIFYQGLQEGAQNKTARLTDEEMAKAINDYKKTLEAKQLVEFQKQGQQNAQAGAAFLAENTKKSGVITTKSGLQYQVLKEGTGKT-PKATSRVKVNYEGRLLDGTVFDSSIARNHPVDFQLN--QVIAGWTKGLQTMKEGGKTRFFIPAKLAYGEVGAGDSIGPNSTLIFDIELLQVLPK 1D7J Chain:A ((1-106)) ---------------------------------------------------------------------------------------------------------------------------------GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKL---

General information: TITO was launched using: RESULT: Template: 1D7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 486 13158 27.07 127.74 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 27.07 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_1D7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1D7J-query.scw PDB file : Tito_Scwrl_1D7J.pdb: