Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRPTVLCFSGLDPSGGAGLQADIEAIGQSGAHAAIACTALTIQNSQQVFGSEATSKELLLAQANAVVG--DLPIKCVKSGMLGTTDNIAALAEFLRAHPDYQYVLDPVLVANSGG-SLGDQATLVKAFVELIL-LATLITPNTVELRALTGVT-----DLDQ---ATQKLFEMGAKAVLVKGGH---EDTPDFIKNSLYIDGELAASSTCPRLEGEYHGSGCSLASFIAGRLALGDSLKIAVQHAETWLFGVLKNAETPVPNGQKIPKRF 3H74 Chain:A ((2-253)) SLSTMLVAEDLSAVGGISLSSALPVLTAMQYDVAALPTSLLSTHTSGYGTPAVVDLSTWLPQVFAHWTRAQLHFDQALIGYVGSVALCQQITTYLEQQTLSLLVVDPVLGDLGQLYQGFD-QDYVAAMRQ-LIQQADVILPNTTEAALLTGAPYQVTPDLEVILPALQAQLKTGAHAVITDVQRADQIG------CAWLDEAGHVQYCGARRLPGHYNGTGDTLAAVIAGLLGRGYPLAPTLARANQWLNMAVAETI-------------

General information: TITO was launched using: RESULT: Template: 3H74.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1219 -48288 -39.61 -206.36 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -39.61 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_3H74.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H74-query.scw PDB file : Tito_Scwrl_3H74.pdb: