Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKTLPLTDIHRHLDGNIRIQTILELGQQYNLDLPAYDIESLRPHVQVMDNQPDLLSFLSKLDWGVKVLASLDACKRIAFENMQDAAQQGLDYVELRFSPGYMGMAHQLPLEGVVEAVIAGVKEGSQAYGVKANLIGIMSRTFGQEACEKELNALLAHKNDIKALDLAGDELGFPGNLFIDHFKKARDAGWHITVHAGEAAGPESIWQAIEELGAERIGHGVKAVQDLKLLDYLAKHQIGIESCLTSNIQTNTVPSLAEHPLKTFLEHGVLATINTDDPAVEGIEIQHEYLTAAPLAGLSPEQIYTAQENGLKIAFLSEQEKDELRQKYQ 3PBM Chain:B ((5-317)) EWLNALPKAELHLHLEGTLEPELLFALAERNRIALPWNDVETLRKA----YAFNNLQEFLDLYYAGADVLRTEQDFYDLTWAYLQKCKAQNVVHVEPFFDPQTHTDR-GIPFEVVLAGIRAALRDGEKLLGIRHGLILSFLRHLSEEQAQKTLDQALPFRDAFIAVGLDSSEVGHPPSKFQRVFDRARSEGFLTVAHAGEEGPPEYIWEALDLLKVERIDHGVRAFEDERLMRRLIDEQIPLTVCPLSNTKLCVFDDMSQHTILDMLERGVKVTVNSDDPAYFGGYVTENFHALQQSLGMTEEQARRLAQNSLDARLV-------------

General information: TITO was launched using: RESULT: Template: 3PBM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1874 -152462 -81.36 -487.10 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -81.36 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_3PBM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PBM-query.scw PDB file : Tito_Scwrl_3PBM.pdb: