Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIKFNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVET-SNLRISDRAHVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERLRLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVLFEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGPFPTELFDE--------TGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVLSGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPENARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI 3UE9 Chain:D ((15-451)) GRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTL------TILRLIPSGIMREGVACYIGNGVVLSPEALFKEIGELEEAGLSVRERLFISEATTLILPYHIAIDQAREAR----------RGIGPAYEDKVGRRALRVQDLFDARTFADRLRENLDFHNFVLTQYLGGAAVDFQATLDTMLGYADRLRPMVADVSRRLYEENHAGRNLLFEGAQGTLLDIDHGTYPFVTSSNCVAGAAAAGAGVGPQKLNYILGITKAYCTRVGSGPFPSELYDADNPSRQDQIGITLANVGKEFGSVTGRPRRTGWLDAAALRRSIQINGVSGLCMTKLDVLDGLDEVKLCVGYKIDGEDADLLPRGAAEVARCEPVYETFGGWKESTVGINSWDALPANARAYLTRVQEVAGVPIDMVSTGPDRDETILLRHPFK--

General information: TITO was launched using: RESULT: Template: 3UE9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2438 -24769 -10.16 -60.12 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain D : 0.84 3D Compatibility (PKB) : -10.16 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_3UE9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UE9-query.scw PDB file : Tito_Scwrl_3UE9.pdb: