Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPELAKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIRGDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK 2HVE Chain:A ((4-136)) CERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGGDSVESAEKEIGLWFHP------------

General information: TITO was launched using: RESULT: Template: 2HVE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 604 -83062 -137.52 -624.52 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -137.52 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.620

(partial model without unconserved sides chains): PDB file : Tito_2HVE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HVE-query.scw PDB file : Tito_Scwrl_2HVE.pdb: