Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVMQLLPELNSGGVERGTLEIARALVAQGHQSLVVSNGGRLVSQLEAEGSTHLTLPIHKKSLSSLWQIRPLRQLIEEHQPDIVHVRSRVPAWLTHFALRKIPANKHPHLISTVHG-----------FYSVNRYSAIMTQAEKVIAVSDSVVKYITDHYKNCPPQDIVRIYRGIDPAAFPHNYQPSAQWFNQVFNDFPELENKFLLCLPGRITRLKGHESLIELMQKL-GEQYPQLHAVVVGGADVKKQTYLSELQNTIQSKGLADKITFVGHRSDIREWLAFSDIVLSLSNQAETFGRTALEALSVGTPVIGWNRGGVAEILSHVYPQGLVEAENEKALVERVKHHIEQPQ-----TVAPVTM----FSLKDMCDQTLELYQSVLK 5D00 Chain:A ((81-375)) -----------------------------------------------------------------------IAEVAERENLDIIHAHYALPHAVCAY-LAKQMLKRNIGIVTTLHGTDITVLGYDPSLKDLIRFA--IESSDRVTAVSSALAAETYDLIK--PEKKIETIYNFI-------KNTAAIKEKHGILPD-----EKVVIHV-SNFRKVKRVQDVIRVFRNIAGKTKAKL--LLVG--DGPEKSTACEL---IRKYGLEDQVLMLGNQDRVEDLYSISDLKLLLSEK-ESFGLVLLEAMACGVPCIGTNIGGIPEVIKNNVSGFLVDVGDVTAATARAMSILEDEQLSNRFTKAAIEMLENEFSSKKIVSQYEQIYADL--

General information: TITO was launched using: RESULT: Template: 5D00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1412 -36270 -25.69 -135.84 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -25.69 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_5D00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D00-query.scw PDB file : Tito_Scwrl_5D00.pdb: