Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGIRITGTGLFHPTEIISNEELADSLNAYVEQYNQENAEKIAAGELEELRGSSAEFIEKASGIKRRYVIEKSGILDPTRLRPRLSERSNDELSIQAEWGVIAAKQAMENAGVTAEDIDVVILACSNMQRAYPAVAIEIQSALGIQGYA-YDMNVACSAATFGLKQAADAIRSGA-RRVLLVNVEITSGHLDYRNRDCHFIFGDVATASIIEETTTKTGFEILDIHLFTQFSNNIRNNFGFLNRSEDAVVDDKLFRQDGRKVFKDVCPLVAKIINAQLEKMQLTANDIKRFWLHQANANMNELILKYVAGKDADLSRTPIILDEFANTSSAGVIIALH---RTGHEVDDGEYGVISSFGAGYSVGSIVVQKHVA 4YLT Chain:A ((1-315)) MYTKILGTGSYLPVQVRSN------------------------ADLEKMVDTSDEWIVTRTGIRERRIAG----LDET----------------VATMGFQAAEKALEMAGIDKDDIGLIIVATTSSSHAFPSSACQVQRMLGIKDAASFDLAAACAGFTYALSVADQYVKSGAVKHAIVIGSDVLSRALDPEDRGTIILFGDGAGAVVLGASEQPG---IMSTHLHADGRYGELLALPYP---------PAYVTMAGNEVFKVAVTELAHIVDETLQVNNLDRTALDWLVPHQANLRIISATAKKLG---MGMDKVVITLDRHGNTSAASVPSAFDEAVRDG-RIQRGQLVLLEAFGGGFTWGSALVR----

General information: TITO was launched using: RESULT: Template: 4YLT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2013 -102885 -51.11 -338.44 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -51.11 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_4YLT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YLT-query.scw PDB file : Tito_Scwrl_4YLT.pdb: