Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKLTKRQKAIAAAVEANKVYTLEEAVQVLNSLPAAKFKESLDISVNLGVDPRKSDQVVRGATTLPAGTGKTVRVAVFAQGAQAEAAKEAGADVVGFDDLAESIQGGNLDFDVVIAAPDAMRVVG-KLGTILGPRGLMPNPKVGTVTPDVAGAVKNAKSGQARYRVDKAGIIHAAIGQVGFDAAAIRQNVETLVADLKKLKPATSKGVYIKKITLSSTMGPGLTVDVNNVSS 4F9T Chain:A ((2-229)) -PKHG--YRALLEKVDPNKIYTIDEAAHLVKELATAKFDETVEVHAKLGIDPRRSDQNVRGTVSLPHGLGKQVRVLAIAKGEKIKEAEEAGADYVGGEEIIQKILDGWMDFDAVVATPDVMGAVGSKLGRILGPRGLLPNPKAGTVGFNIGEIIREIKAGRIEFRNDKTGAIHAPVGKASFPPEKLADNIRAFIRALEAHKPEGAKGTFLRSVYVTTAMGPSVRINPHS---

General information: TITO was launched using: RESULT: Template: 4F9T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1184 -22127 -18.69 -98.34 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -18.69 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_4F9T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F9T-query.scw PDB file : Tito_Scwrl_4F9T.pdb: