Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTLAEAYRQFDLTRLIEEQLVGHPQAINAYVECCERYVGKNKTALIWEGKNGQAEYYTFEQLAELSGKLANFFK-AQGIQAGDCIAGLLPRTPELLITILAAWRIGAIYQPLFTAFEAKSIDHRITTAQTKLIVTNDEQRPKLNSLNVPVIVTVHQTGLLSEHDFDFWQSLQRYSEQCEPVNRSVD----DDFLMMFTSGTTGLAKSVPVPLKAILAFKGYMTHA-VDLREEDMFWNLADPGWAYGLYYGITGPLSLGHSIIMDER-SFNVDQAMELIKKYKVSNLTGSPTAFRMFFGFKEKFDPSIKQHLRVVSSAGEPLTPEVVNWFKQDLEVNIFDQYGQTELGMVIANHHALEHPLKVGSAGFAIPGHRFAVLDQNYQELPTGGVGILAMDSEQ-SPLMWFKGYGGNNRKSFV---GKYYLTGDTVTLNEHGGIGFVGRADDVITTSGYRVGPFDVESTLLECEAVLESAVIGKPDPERTEIVKAFVVLKPAYQA--CETLKDKLQQYVRNRLSRHAYPKEIEFVDSLPKTTSGKIQRGLLKQQEIAKMQPYAS 2VZE Chain:A ((61-560)) --------------------------------------------ALWWVNGKGKELMWNFRELSENSQQAANVLSGACGLQRGDRVAVVLPRVPEWWLVILGCIRAGLIFMPGTIQMKSTDILYRLQMSKAKAIVAGDEVIQEVDTV-ASECPSLRIKLLVSEKSCDGWLNFKKLLNEASTTHHCVETGSQEASAIYFTSGTSGLPKMAEHSYSS-LGLKAKMDAGWTGLQASDIMWTISDTGWILNILCSLMEPWALGACTFVHLLPKFDPLVILKTLSSYPIKSMMGAPIVYRMLL--QQDLSSYKFPHLQNCVTVGESLLPETLENWRAQTGLDIRESYGQTETGLTCMVSKTMK--IKPGYMGTAASCYDVQIIDDKGNVLPPGTEGDIGIRVKPIRPIGIFSGYVDNPDKTAANIRGDFWLLGDRGIKDEDGYFQFMGRADDIINSSGYRIGPSEVENALMEHPAVVETAVISSPDPVRGEVVKAFVVLASQFLSHDPEQLTKELQQHVKSVTAPYKYPRKIEFVLNLPKTVTGKIQRAKLRDKE---------

General information: TITO was launched using: RESULT: Template: 2VZE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2882 -734 -0.25 -1.51 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -0.25 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_2VZE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VZE-query.scw PDB file : Tito_Scwrl_2VZE.pdb: