Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTKKDLLQQRMLIDQLRTQNSLSPQNAAKLGQSIASSWERSASAAIPKERFAAPLLEKKSASQNALDLALSQCADDLRHIAEQSSMVIAVGDIGSTIIWTASSAQMQSAAERVHFVQGGQWREEFVGTNALALSLKTQQSSCVFSNEHYMESIHDWVCYAAPIIDPYSKQTLGVVDLSTTWKNHNTLGILAAERCASIIQSALLEQQRQQLHIRAFSTPQVKFNGKSLL-LTPRQIEILTILALCP-HGLTLENLYQALYGERK--VSMGTLKAEMSQLRDILGGL---------LGSRPYRLLVH-VEADFLQAEQALDAGY--------------VASALQLYAGVFLAKT-ESPFLCAWRDCLESRLSDA-------IFKAQETDLLLKHLAHF----PEAIDAVERLMELLP-SEHPAHQLLVKYIDSPKLS 2FF4 Chain:B ((12-225)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DFGLLGPLQMTIDGTPVPSGTPKQRAVLAMLVINRNRPVGVDALITALWEEWPPSGARASIHSYVSNLRKLLGGAGIDPRVVLAAAPPGYRLSIPDNTCDLGRFVAEKTAGVHAAAAGRFEQASRHLSAALREWRGPVLDDLRDFQFVEPFATALVEDKVLAHTAKAEAEIACGRASAVIAELEALTFEHPYREPLWTQLITAYYLSDRQSDAL-----------

General information: TITO was launched using: RESULT: Template: 2FF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 719 -30087 -41.85 -173.91 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -41.85 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_2FF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FF4-query.scw PDB file : Tito_Scwrl_2FF4.pdb: