Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKQTVQLEIISVNQTKTKIELVISQIEQQIKNRSLTPGTRLPSVRKLAKDLGFSVSTIVEAYERLIALGKIESRSGSGFYVVAPLAPLALNELGPKLDRSIDPLWISRQSLEAEPNAFKPGCGWLPN-DWMPLESIRKALRSATRSEDDSLLGYSSPLGLSALRDLLARRAQSK-GIEANLNQVLLTDSGTQAIDLVCRFLLKPDDVVLIDDPCYFNFHALLKVHQVKVVGIPYTPNGPDLEAFKEAI-----EGYNPRLYITNSGIHNPTGATLSLSTAHQLLKLIDQSNLIVIEDDIFSDFEYT--PAPRLAALDNLSRVIFIGSFSKTLSASIRCGYIIAKPEWIDQIADLKIATSFSHNGLSAKILHTALTDGSYRKH----LDLLKVRLAQAMQETIAKLKSIRIEPWIEPKAGIFVWCRLPEGVDAEKIAQFCLNRQVILAPGNAFSQAQSAGQFIRFNITQSNHDYI---YKTLAEALLQESLEKQV 1WST Chain:A ((47-411)) --------------------------------------------------------------------------------------------------------------------------AGGLPAPETFPVETIKKIAVEVLEEHADKALQYGTTKGFTPLRLALARWMEKRYDIPMSKVEIMTVAGSQQALDLIGRVFLNPGDPIVVEAPTYLAAIQAFKYYDPEFISIPLDDKGMRVDLLEEKLEELRKQGKRVKIVYTVSTFQNPAGVTMSVDRRKKLLELANEYDFLIVEDGPYSELRYSGEPTPPIKHFDDYGRVIYLGTFSKILAPGFRIGWVAAHPHLIRKMEIAKQSIDLCTNTFGQAIAWKYVENGYLDEHIPKIIEFYKPR-RDAMLEALEEYMPEGVE-WTKPEGGMFVRVTLPEGIDTKLMMERAVAKGVAYVPGEAFFVHRDKKNTMRLNFTYVPEETIREGVRRLAETIKEE------

General information: TITO was launched using: RESULT: Template: 1WST.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1959 -11328 -5.78 -32.46 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -5.78 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_1WST.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WST-query.scw PDB file : Tito_Scwrl_1WST.pdb: