Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIAKLKIAIIGLGYVGLPLAVEF---GKKVPVVGFDIYQKRIDELKNGQDHTLEVSPEE-LKQAV---HLKYTAHLDDL---QDSNFFIVTVPTPIDDFKQPDLTPLIKASTSIGQVLK---KGDVVVYESTVYPGATEE-VCIPVLEKISGLKFNQDFFAGYSPERINPGDKQHRVTNILKITSGSTPE-VADYIDEVYNLIIEAGTHKAPNI----KVAEAAKVIENTQRDVNIALINELALIFNKLDIDTEDVLKAAG--TKWNFLPFRPGL-VGGHCIGVDPYYLTHKAQSIGLHPEIILAARRLNDRMGEHVATQLIKEMVKQRIQVVGARILVMGLSFKENCPDIRNTKIVDFIKALKEYDLDLDIYDPWVDDTEVQHEYGLAPIKELQQGLYDAIVIAVAHNQFKSMSVEEFHALGKEKHVLYDLKYVLDKESSNIRL 3VTF Chain:A ((23-374)) ------SLSVLGLGYVGVVHAVGFALLGHRV--VGYDVNPSIVERLRAGRPHIYEPGLEEALGRALSSGRLSFAESAEEAVAATDATFIAVGTPPAPDG--SADLRYVEAAARAVGRGIRAKGRWHLVVVKSTVPPGTTEGLVARAVAEEAGGVKFS----VASNPEFLREGSALEDFFKPDRIVIGAGDERAASFLLDVYKAV------DAPKLVMKPREAELVKYASNVFLALKISFANEVGLLAKRLGVDTYRVFEAVGLDKRIGRHYFGAGLGFGGSCFPKDTLAFIRFGESLGLEMAISKAVLRVNEYMPRY-AVQLLEERLGG---LRGRHVGVLGLAFKPNTDDVRESRGVEVARLLLERGARVYVHDP----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3VTF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1685 -13666 -8.11 -41.41 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -8.11 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_3VTF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VTF-query.scw PDB file : Tito_Scwrl_3VTF.pdb: