Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTNLAHKQEEDNVITLHPSTAKKKERTAMSDKFDKGYVMSSR-LYRNEVKPFLGDAARNVYAELEEYIS-----GFN--KESDFV--SYSQLQGKKIEGLEEHVRKLSTATVRAGLKQLIEYGVISIVATNPKL-GNKYKLNEISLVEHFSNKSTSETKALQKLNSTTLETKAQGTLETKDTIDIIYRYLIIDNLFNSLRSNKPLENHFFVYQENKKQVFLEQQKLEAEEKAKAEKERKDKVRKLSFDEVIKLTKTTFANLCDLELWEQYVAFRSQKANTKLTKNALNVIYKDLKAWGFQGSNQSLKTAISGNYLGLFAPKQQNHGFVNQSQASTRMSEIQELIAKEEAGNEQYGF 4PQL Chain:A ((10-114)) --------------------------------NYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRDRLNLSIKNSWVDEDGNIYFVYSNEKLMEI---------LNCKKEKLTKIKKGLENDGLLIQKRRGLNKPNILYLMKPIV-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4PQL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 245 9172 37.44 97.57 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 37.44 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_4PQL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PQL-query.scw PDB file : Tito_Scwrl_4PQL.pdb: