Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPGSVFFDQQFSFHDGE--SGEKLFVILGWD--N--------GIAIVAKTTSQQHGRGTTFGCQPKDRFHNFYLPQNSCYFRKCTWVCLDEFYELNAVEVLQKRFSGLINPVCNLTNEMLRKLQDCALESDDLSGRQESIIRSSLV 5HJZ Chain:B ((3-115)) MRRGEIWQVD-LDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKV--YPFQ--------VLLSATT-GLQVDCKAQAEQIRSIATERLL--------RPIGRVSAAELAQLDEALKLHL---------------

General information: TITO was launched using: RESULT: Template: 5HJZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 456 -33385 -73.21 -333.85 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -73.21 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.598

(partial model without unconserved sides chains): PDB file : Tito_5HJZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HJZ-query.scw PDB file : Tito_Scwrl_5HJZ.pdb: