Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRVAKAPVTVPNGVTVTQNGRQVEVKGSKGTLSFNLHALVELKQEEGKLQLAPAKESKDAWMQAGTARAVLNNLVKGVSEGFERKLQLVGVGYKAVVKGTVVNLNLGYSHPIDYALPEGVTAETPTATEIILKSANKQLLGQVAAEIRAYRSPEPYKGKGVRYSDEVILRKEAKKK 2J28 Chain:G ((1-176)) -SRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAKKK

General information: TITO was launched using: RESULT: Template: 2J28.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 763 -57613 -75.51 -327.35 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain G : 0.88 3D Compatibility (PKB) : -75.51 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_2J28.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J28-query.scw PDB file : Tito_Scwrl_2J28.pdb: