Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMTEQKSALSLRYFLNLEESQDGFGLVTFGKKQFTRFLTPVISIAIIIWGLYLGFSGVGRYYVALGVFFLVMQGLMRYWLLPMMFKRQFVRYQFGKSEQGIDLY--QDYFEFYAAGRKQAVQYSEVQTFAKGKL-------TYMLELKNKTVIIVPKRAFAQASDQTIFENTFKK 4CHJ Chain:A ((63-146)) --------------------------------------------------------------------------------------------------CNLKIDRPKNFMNLTFNQKVRPIQLDDIAAVLYG--SKMLRNPVVGFRLAS-SGRAIAFS-FKDITDAQCFVSFLDD

General information: TITO was launched using: RESULT: Template: 4CHJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 185 -443 -2.39 -6.92 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : -2.39 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.255

(partial model without unconserved sides chains): PDB file : Tito_4CHJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CHJ-query.scw PDB file : Tito_Scwrl_4CHJ.pdb: