Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFTRINGVLHAEQCSLEQLAQQFGTPLYVYSKAAFEKHYLDMDRAFDFIDHQ------ICFAVKSNSNIAVLNVLAKLGAGFDIVSGGELARVLKAGGDASKIVFSGLGKTEADIETSLNVGIACFNVESYAELDRIQKVAARLGKKAPISLRVNPDVDAKTHPYISTGLKENKFG--IPSDAVYETYQYAASLPNLEIVGIDCHIGSQLTETKPFVDALDRVIVMIDELKKLGINLKHIDIGGGLGV-CYKDETPPSVEEYANSMKPALEKLGLKVYM-----EPGRSISANAGVLLTKVDLLKPTTHRNFAIIDAAMNDLIRPALYEAWMDIQPVVPRTDTEEKTWDLVGAICETGDFIGKDRSL-ALQENDLLTVLGAGAYGFVMSSNYNTRGRAAEVMVSGEKSYLIRERETVESLWEKERLLPEE 1TUF Chain:A ((23-431)) ------------------ELAEKFGTPLYVMSEEQIKINYNRYIEAFKRWEEETGKEFIVAYAYKANANLAITRLLAKLGCGADVVSGGELYIAKLSNVPSKKIVFNGNCKTKEEIIMGIEANIRAFNVDSISELILINETAKELGETANVAFRINPNVNPKTHPKISTGLKKNKFGLDVESGIAMKAIKMALEMEYVNVVGVHCHIGSQLTDISPFIEETRKVMDFVVELKEEGIEIEDVNLGGGLGIPYYKDKQIPTQKDLADAIINTMLKYKDKVEMPNLILEPGRSLVATAGYLLGKVHHIKETPVTKWVMIDAGMNDMMRPAMYEAYHHI--INCKVKNEKEVVSIAGGLCESSDVFGRDRELDKVEVGDVLAIFDVGAYGISMANNYNARGRPRMVLTSKKGVFLIRERETYADLIAKDIVPP--

General information: TITO was launched using: RESULT: Template: 1TUF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2345 60067 25.61 152.45 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : 25.61 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_1TUF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TUF-query.scw PDB file : Tito_Scwrl_1TUF.pdb: