Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIAIIGAGISGLMSALELVEQGCTISIFDQQQ-AGQAASWAGGGILSPMYPWRYVHAVNQLAQFGKASYQAWNQKLYPVTGIDFEIHDTGM--LIFDEEDFDVGLSYAEQHQEPIQRCEYLQRDALEQVNPHISEQFQEAIY------FPELSNIRNPRVLQSLISYLKQHPNVEFFEHAAVKKLIQQGDVIQALQTEDGRKHTADHFVITSGAWSHYWNSQLKLEIPVEPV-QGQMLLFKTPAHWLPTMCMNRVMYLIPRMDGHI-----VCGSSMAHRGFD-------------------------TSTDETTQHNILE----------ACLEMVPEL--------ADFP------IVHRWAGLRPSSPNGVPYIGKMPEMDNLWANFGHFRNGLCMGAGSAQLLRQLMLGQPTLVDAKAYSPERLQNKILA 5HXW Chain:A ((40-454)) --VVVVGAGILGIMTAINLVERGLSVVIVEKGNIAGEQSSRFYGQAIS----YKMPDETFLLHHLGKHRWREMNAKV----GIDTTYRTQGRVEVPLDEEDLVNVRKWIDERSKNVGSDIPFKTRIIE--GAELNQRLRGATTDWKIAGFEEDSGSFDPEVATFVMAEYAKKMGVRIYTQCAARGLETQAGVISDVVTEKGAIKTS-QVVVAGGVWSRLFMQNLNVDVPTLPAYQSQQLISGSPTAPGGNVALPGGIFFREQADGTYATSPRVIVAPVVKESFTYGYKYLPLLALPDFPVHISLNEQLINSFMQSTHWNLDEVSPFEQFRNMTALPDLPELNASLEKLKAEFPAFKESKLIDQWSGAMAIAPDENPIISEVKEYPGLVINTATGW-GMTESPVSAELTADLLLGKKPVLDPKPFSLYRF------

General information: TITO was launched using: RESULT: Template: 5HXW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1727 -27099 -15.69 -77.20 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -15.69 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.304

(partial model without unconserved sides chains): PDB file : Tito_5HXW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HXW-query.scw PDB file : Tito_Scwrl_5HXW.pdb: