Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQTLIFMHETGKISIDFFKECIMQDIPMSRWLSPLMAFCLSFVIMATLAPTMGIQIDRQIDFWLLWFGTMLLLALPVCYLEIALAKRSKTTALNALSSLTREADSSPKWRVVGCLAVVFIPFLA------------------GNVLSTA------------------------------SNILVAQ----------FAPS-ISSQI-----IFVGLAVAALVLSFIP--R--QILILLMTLGVIASIVLANMM-GS-----TLQPWHWTSV-EFKEWGNATVLALVASGLGLGLYW-QNSVGAVQAQEGATKTVLPIWLAQLIAVVAFGFFSL---------------------------------QAQLPVLTW---------IFTGVMTSALFVQLAREQLAQRQLMP---VLQWVIIVVAIAVWAVPEVHNL------FTLIL-MQ--WGL-LICLIYAVFAGWIMKISHLRKSMNFSN-ELFYNLWRIAVRIVLPLSIIVAMIAVIGQSI 5I6X Chain:A ((30-484)) ----------------------------------------------WRFPYICAQ-NGGGAFLLPYTIMAIFGGIPLFYMELALGQYHRNGCISIWRKI------CPIFKGIGYAICIIAFYIASYYNTIMAWALYYLISSFTDQLPWTSCKNSWNTGNCTNYFSEDNITWTLHSTSPAEEFYTRHVLQIHRSKGLQDLGGISWQLALCIMLIFTVIYFSIWKGVKTSGKVVWVTATFPYIALSVLLVRGATLPGAWRGVLFYLKPNWQKLLETGVWIDAAAQIFFSLGPGFGVLLAFASYN--KFNNNCYQDALVTSVVNCMTSFVSGFVIFTVLGYMAEMRNEDVSEVAKDAGPSLLFITYAEAIANMPASTFFAIIFFLMLITLGLDSSFAGLEGVITAVLDEFPHVWAKRRERFVLAVVITCFFGSLVTLTFGGAYVVKLLEEYATGPAVLTVALIEAVAVSWFYGITQFCRDVKEMLGFSPGWFWRICWVAISPLFLLFIIASFL----

General information: TITO was launched using: RESULT: Template: 5I6X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1239 -203011 -163.85 -628.52 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -163.85 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.234

(partial model without unconserved sides chains): PDB file : Tito_5I6X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I6X-query.scw PDB file : Tito_Scwrl_5I6X.pdb: