Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPVVHAFRASQSGLDAGAMKRSSDSHSPPFFFLSSACLAYRTSPMPSPRFARPELNGIITVRRFQRPQLETEAPIRKGDLKEPLEAVAGRVGLRARCSTSAPSADLCLVDDHELVLMGKLVDKGVYYITGGVCIDAEGSDTSSQSPTAALVSSTACGAAPQPPDITVGWCFDARMLLHRSDMN-RSPETPHRLQRAIETLQGCERALDVLPVELLAPFTVHKDSSSGSDLGRAVPPISAANRSQWIPARLATYDEVCSFQDPQVYEHFLKSGAAL----VNLKSD----VYCNDETSSVAVRLSAGAVIDASVAALRGVAASRSGTASSAAGRVHPLVSFCLVRPPGHHCTASQPSGFCLVNNVAIAVQQLRIRHASALPSGPPRIAILDLDVHFGEGTASFVEGACDPASLLYLSLHRYDKRQFYPFDGRGDTAYVGGSRHAASKGSICNVAVHTNGQQPARCEQVISDHLMNSVLEEIFLPRLAEFGPDLVMVSLGFDAAYGDPLGKMAVE-GGFASVLSRLKWWCLHDGRTAGLVVVLEGGYNPEAVAQGV---LSVALALSLPRTDPL---------LQRFLEEKSPKVWSDLRQRQERRHREWQQLREERAEEDVGAAPSGLGSELKPAASDEPEQVQEDALLLDRHKRWCAALVAKVQQIHREAMTRER 5EEN Chain:B ((8-364)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------P-----ITGLVYDQRMMLHHNMWDSHHPELPQRISRI---------------------FSRHEE-------------LRLLSRCHRIPARLATEEELALCHSSK-HISIIKSSEHMKPRDLNRLGDEYNSIFISNESYTCAL-LAAGSCFNSAQAILTGQV--RNAVA--------------IVRPPGHHAEKDTACGFCFFNTAA-----LTARYAQSITRESLRVLIVDWDVHHGNGTQHIFE---EDDSVLYISLHRYEDGAFFPNSEDANYDKVG-----LGKGRGYNVNIPWNGGKMGDPEYMAAFH-------HLVMPIAREFAPELVLVSAGFDAARGDPLGGFQVTPEGYAHLTHQLM--SLAAGR---VLIILEGGYNLTSISESMSMCTSMLLGDSPPSL---PLKTSATVSINNVLRAHAP-FWSSLR-----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EEN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2131 -29201 -13.70 -88.22 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -13.70 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_5EEN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EEN-query.scw PDB file : Tito_Scwrl_5EEN.pdb: