Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQAKGEAAMRDLIAELHAMQSPYTVQRFISSGSYGAVCAGVDS-EGIPVAIKRVFNTVSDGRTVNILSDSFLCKRVLREIRLLNHFHHPNILGLRDIFVHFEEPAMHKLYLVTELMRTDLAQVIHDQRIVISPQHIQYFMYHILLGLHVLHEAGVVHRDLHPGNILLADNNDITICDFNLAREDTADANKT----HYVTHRWYRAPELVMQFKGFTKLVDMWSAGCVMAEMFNRKALFRGSTFYNQLNKIVEVVGTPKLEDVVMFSSPSARDYLRNSLSNVPARAWTAVVPTADPVALDLIAKMLEFNPQRRISTEQALRHPYFESLFDPLDLTEGLSER-FHFDESVTDV--WDMHKIFTAEVERFNDLRERREEVARERAMAAQQQGEQVLGTDHMPRTHSLMELAGNAPAPS 4FV2 Chain:A ((25-354)) ----------------------YTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKI----------SPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHL--SNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLL-SLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSD--EPIAEAPFKF--ELDDLPKEKLKELIFEETARF------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4FV2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1617 -46792 -28.94 -146.22 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -28.94 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.597

(partial model without unconserved sides chains): PDB file : Tito_4FV2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FV2-query.scw PDB file : Tito_Scwrl_4FV2.pdb: