Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence TVASRRDDCWVSLFQYKANGEREAIYVDYSPPNSLVIFVHDGWRLN------VQLDQHCRSL-FPNDIRKQLVEGFGVHSYSKMNEVAGQKPLARSQ 1ECZ Chain:B ((12-73)) --------------PYPQAEKGMKRQVIQLTPQE----DESTLKVELLIGQTLEVD--CNLHRLGGKLENKTLEGWGYDYYV---------------

General information: TITO was launched using: RESULT: Template: 1ECZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 39 -452 -11.59 -8.22 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -11.59 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_1ECZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ECZ-query.scw PDB file : Tito_Scwrl_1ECZ.pdb: