Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQEFINPSDGV----IRQYLATSKTLAVVGLSDREETTSNRVTKEMQARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDF----LKADAKIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA 3QA9 Chain:A ((3-140)) -------PIDGLTDEDIREILTRYKKIALVGASPKPERDANIVMKYLLEHGYDVYPVNPNYE--EVLGRKCYPSVLDIPDKIEVVDLF----VDPAKAWRFVVYAIKKGAKVVWFQYNTYYPLAARQAKEAGL-IIVANRCMMREHERLLGE-

General information: TITO was launched using: RESULT: Template: 3QA9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 695 3871 5.57 29.77 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 5.57 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_3QA9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QA9-query.scw PDB file : Tito_Scwrl_3QA9.pdb: