Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVKFSMEKENRGKNMAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSITVVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNKLVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRFKLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYSETYKRSDYYKIKFISCDRDH 4ZSI Chain:A ((29-161)) -------------------------------------------------------------------------------------------QLLDIGYITADDRLAGLLDITAGGRVL--------------RIERLRMANGEPMAIETTHLSAKRFPALRRSLVKYTSLYTALAEVYDVHLAEA--EETIETSLATPREAG-LLGTDVGLPMLMLSRHSQ-DRTGQPVEWVRSVYRGDRYK----------

General information: TITO was launched using: RESULT: Template: 4ZSI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 606 -45512 -75.10 -342.20 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -75.10 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_4ZSI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZSI-query.scw PDB file : Tito_Scwrl_4ZSI.pdb: