Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFVWYNLFLKKEREVISMRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVYDSRTEKLTFGKEVENLEITLHQHTLTITDSFD-DQIHISYHPSLSAHHDLITNQNDRTLSLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTIIN-ASLENATLNTNS-YILRIEG-SRIKNSKLT--TPNIVNIFDTVLTDSQLES-TENHFHAENIQVHGKVELTA-K-DYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYKTEKTDVKDQLIARSDDNIDLISTPSRR 3JX8 Chain:A ((14-195)) ---------------------------------------------------------------ETREIKI-SDYDEITFVG-SADFEYEQSDKAPYLSVTIDE--NLFDYLVTEVEGGTLKIYPKSIKKG-FNN---NS----YD-LRPTVYKIKSNSK---ELKELNTV-GSGSFIISKPTKVNRMEINMAGSGNVELRGPVKGYKLECNMAGSGNIIAKDIQLDNLSCSLASSGEIEVIGT--VDRASFNVAGSGEIKAFDC-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3JX8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 956 63118 66.02 364.84 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 66.02 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.212

(partial model without unconserved sides chains): PDB file : Tito_3JX8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JX8-query.scw PDB file : Tito_Scwrl_3JX8.pdb: