Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTMQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHIIGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILKLLKIQLIHCHGYSTNYFL--YMLKKLDKNGFGKVKTVITCHGW----VEYNLKKKFLTYFDFWTYSMGDAFICVSETMKKRLESIIK-NKKIVAINNGINVSNSDLDVV-GVQDFKKEFCIPNNKKIICYVGRLDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICHLKCRDNFRLLGEIYPATIVYQISDLLYIPSDTEGIPMCVLESMSQGTSVLASNVGGLSEIIEHRVDGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHFSVQKMFVETMRVYDELLEKSSHG 5D01 Chain:A ((90-376)) ------------------------------------------------------------------------------------------------------------LDIIHAH-YALPHAVCAYLAKQMLKRNIG---IVTTLHGTDITVLGYDPSLKDLIRFAIES---SDRVTAVSSALAAETYDLIKPEKKIETIYNFI----------KNTAAIKEKHGILPDEKVVIHVSNFRKVKRVQDVIRVFRNI-AGKTKAKLLLVGDGPEKSTACELIRKYGLEDQVLMLGNQ--VEDLYSISDLKLLLSEKESFGLVLLEAMACGVPCIGTNIGGIPEVIKNNVSGFLVDVGDVTAATARAMSILEDEQLSNRFTKAAIEMLENEFSSKKIVSQYEQIYADLA------

General information: TITO was launched using: RESULT: Template: 5D01.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1440 -8158 -5.66 -30.21 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -5.66 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_5D01.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D01-query.scw PDB file : Tito_Scwrl_5D01.pdb: