Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIAVIGLGHVGLAYALLFAS-KYKVVAYDIDSVKINNLKKGILPSKNEELMKFFCEN--NLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVSKILR--VKPNGKIILKSTVPIGFSNKLKRLF------DTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIA-ELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTKQLKKEFIEINAP--VIEAIDISNTNRKQFIVKQILER-----KPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIKILVYEP----NLNVSIDNVIFEKNFELFTKQSD-LIVANRWDRGLEAYKDKVYTRGIWIRD 4A7P Chain:A ((9-399)) VRIAMIGTGYVGLVSGACFSDFGHEVVCVDKDARKIELLHQNVMPIYEPGLDALVASNVKAGRLSFTTDLAEGVKDADAVFIAVGTPSRRGDGHADLSYVFAAAREIAENLTKPS-VIVTKSTVPVGTGDEVERIIAEVAPNSGAKVVSNPEFLREGAAIEDFKRPDRVVVGTEDEFARQVMREIYRPLS-----P---VLFTGRRTSELIKYAANAFLAVKITFINEIADLCEQVGADVQEVSRGIGMDNRI---FLHAGPGYGGSCFPKDTLALMKTAADNETPLRIVEATVQVNDARKRAMGRKVIKAMGGDVRGKTVGILGLTFKPNTDDMRDAPSLSIIAALQDAGATVKAYDPEGVEQASKMLTDVEFVENPYAAADGADALVIVTEWD-------------------

General information: TITO was launched using: RESULT: Template: 4A7P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1844 -356 -0.19 -0.99 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -0.19 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_4A7P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4A7P-query.scw PDB file : Tito_Scwrl_4A7P.pdb: