Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILITGANGQLGTELRYLLDERNEEYVAVDVAEM----DITDAEMVEKVFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTDYVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKNFVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTY---LAENRKEF-GYYHLSNDATEDTTWYDFAVEILKDTD--------VEVKPVDSSQFPAKAKRPLNSTMSLAK-AKATGFVIPTWQDALQEFYKQEVR 1N2S Chain:A ((3-283)) -ILLFGKTGQVGWELQRSLAPVGN-LIALDVHSKEFCGDFSNPKGVAETVRKLRPDVIVNAAAHTAVDKAESE-PELAQLLNATSVEAIAKAANETGAWVVHYSTDYVFPGTGDI--PWQETDATSPLNVYGKTKLAGEKALQDNCPKHLIFRTSWVYAGKGNNFAKTMLRLAKERQTLSVINDQYGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAGGT--TTWHDYAALVFDEARKAGITLALTELNAVPTSAYPTPASRPGNSRLNTEKFQRNFDLILPQWE------------

General information: TITO was launched using: RESULT: Template: 1N2S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1438 18884 13.13 71.53 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 13.13 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_1N2S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N2S-query.scw PDB file : Tito_Scwrl_1N2S.pdb: