Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSEKSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAEFALGRSAGVSAIKT----FGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFGKLFQLGGTGD------------YAQLFTSIISNPA-------IALGAQATFIL---LNIFIVSRGVQKGIERASKVMMPLLFIIFVVIIERS--LSLPN--AMEGVLYFLKPDFSKLTSAGL-LYALRQSFFALSLGVTAMLTYASYLDKKTNLVQSRISIVAMNI--------SVSIMAGLAIFQARSPFNIQSEGGPSLVFIVLPQLFDKMPFGT----IFYVLFLFATVTSSVVMLEINVDNITNQDNSKRAKWSVILGILTFVFGIPSALSYGVMADVHIFGKAFFDAMDFLVSNLLMPFGALFLSLFTGYIFKKALAMEELHLDERAWKQGLFQV 2A65 Chain:A ((5-437)) ----------------------------------------------REHWATRLGLILAMAGNAVGLGNFLRFPVQAAENGGGAFMIPYIIAFLLVGIPLMWIEWAMGRYGGAQGHGTTPAIFYLLWRNRFAKILGVFGLWIPLVVAIYYVYIESWTL-----GFAIKFLVGLVPEPPPTDPDSILRPFKEFLYSYIGVPKGDEPILKPSLFAYIVFLITMFINVSILIRGISKGIERFAKIAMPTLFILAVFLVIRVFLLETPNGTAADGLNFLWTPDFEKLKDPGVWIAAVGQIFFTLSLGFGAIITYASYVRKDQDIVLSGLTAATLNEKAEVILGGSISIPAAVAFFGVANAVAIAKAGAFNLGFITLPAIFSQTAGGTFLGFLWFFLLFFAGLTSSIAIMQPMIAFL--EDELKLSRKHAVLWTAAIVF---------FSAHLVMFLNKSLDEMDFWAGTIGVVFFGLTELIIFFWIFGADKAWEEIN-------------

General information: TITO was launched using: RESULT: Template: 2A65.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2131 -239771 -112.52 -617.97 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -112.52 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_2A65.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A65-query.scw PDB file : Tito_Scwrl_2A65.pdb: