Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPFGQLNLFEEESPSEKDGDVPS 1U2U Chain:B ((1-29)) --EVQALKKRVQALKARNYALKQKVQALRHK---------------------------------

General information: TITO was launched using: RESULT: Template: 1U2U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 14 -699 -49.93 -24.10 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -49.93 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_1U2U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U2U-query.scw PDB file : Tito_Scwrl_1U2U.pdb: