Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKIVAIWAQDEEGLIGKENRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGGKQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTIQYRKRKEV 3JWC Chain:A ((8-159)) ---RVSFMVAMDENRVIGKDNNLPWRLPSELQYVKKTTMGHPLIMGRKNYEAIGRP-LPGRRNIIVTRNEGYHVEGCEVAHSVEEVFELCKNEEE-IFIFGGAQIFDLFLPYVDKLYITKIHHAFEGDTFFP-EMDMTNWKEVFVEKGLTDEKNPYTY----------

General information: TITO was launched using: RESULT: Template: 3JWC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 661 -111293 -168.37 -732.19 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -168.37 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.625

(partial model without unconserved sides chains): PDB file : Tito_3JWC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JWC-query.scw PDB file : Tito_Scwrl_3JWC.pdb: